{"product_id":"ypd-yepd-broth-vekstmedium-pose-for-0-5l","title":"YPD (YEPD) broth growth medium - pouch for 0.5L","description":"\u003cdiv class=\"textBodyText\"\u003e\n\u003ch2\u003eYPD Growth Medium\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"textBodyText\"\u003eYPD is used for the growth of Saccharomyces cerevisiae. It is a nutrient-rich medium containing dextrose, peptone, and yeast extract. Dextrose is the energy source. Yeast extract and peptone provide carbon, nitrogen, minerals, vitamins, trace elements, and other essential growth nutrients that stimulate yeast growth.\u003c\/div\u003e\n\u003cdiv class=\"textBodyText\"\u003e \u003c\/div\u003e\n\u003cdiv class=\"textBodyText\"\u003eOne sachet is dissolved in 500ml of water and sterilized in an autoclave. Homebrewers can use a microwave to disinfect the contents in a borosilicate bottle with the lid slightly loose. Avoid superheating. Remove from the microwave, place aluminum foil around the bottleneck. When the bottle has cooled down to approximately 70℃, you can screw on the cap (not too tightly).\u003c\/div\u003e\n\u003cdiv class=\"textBodyText\"\u003e \u003c\/div\u003e\n\u003cdiv class=\"textBodyText\"\u003eYPD medium is commonly used in microbiology in combination with agar to create agar plates, a useful tool for investigating contamination, for starting a culture, isolation, growth experiments, cell counting, and as an indicator of phenotypic characteristics (what an organism does visually differently from another organism).\u003c\/div\u003e\n\u003cdiv class=\"textBodyText\"\u003e \u003c\/div\u003e\n\u003cdiv class=\"textBodyText\"\u003e\u003cstrong\u003eEquipment for making agar growth medium in a petri dish, also called an agar plate:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"textBodyText\"\u003e\n\u003cul\u003e\n\u003cli\u003eBorosilicate bottle 500ml\u003c\/li\u003e\n\u003cli\u003eYPD\u003c\/li\u003e\n\u003cli\u003eAgar\u003c\/li\u003e\n\u003cli\u003ePetri dish\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"page\" title=\"Page 1\"\u003e\n\u003cdiv class=\"layoutArea\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eMethod - enough for pouring 20 agar plates with 9 cm diameter\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eWeigh 10 g agar (2%) together with 25g YPD medium and let swell for 15 minutes in 500 mL room temperature tap water in a borosilicate bottle.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDisinfect (boil) the mixture in a microwave oven, avoiding superheating - screw the lid on so that it gives a little.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGently mix the solution, avoiding air bubbles, and cool the bottle to approximately 60 ◦C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDistribute the mixture evenly into 20 agar plates. 25 mL per plate, or to a height of just under 0.5 cm\u003c\/p\u003e\n\u003cp\u003eWhen pouring the mixture into the dishes, it is enough to fill the dish halfway, then carefully place the lid over it and swirl the dish to distribute the solution evenly.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLet the dishes solidify overnight. The next day, they are ready for use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eRead our \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0621\/8422\/6914\/files\/hvordanbrukeypedogandrevekstmedium-21.pdf\" target=\"_blank\"\u003earticle\u003c\/a\u003e for the use of agar plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"VWR","offers":[{"title":"Default Title","offer_id":41901958561890,"sku":"ICNA114001065","price":259.0,"currency_code":"SEK","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0621\/8422\/6914\/files\/fea85682afdecfb9f666d4624c592f6f-image.jpg?v=1746177455","url":"https:\/\/brewshop.se\/en\/products\/ypd-yepd-broth-vekstmedium-pose-for-0-5l","provider":"Brewshop","version":"1.0","type":"link"}